ELISA (Enzyme-linked Immunosorbent Assay)

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USMLE Notes & Questions Bank

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Hey Friends,

ELISA, short for 'Enzyme-linked Immunosorbent Assay', is a powerful technique to detect substrates (e.g. an antigen). There are multiple subtypes and variants of ELISA. Here, the most common Sandwich ELISA is explained.

More about the DIRECT ELISA:
https://youtu.be/ohIDilaG16I

More about the INDIRECT ELISA:
https://youtu.be/Yfv7FtvozOg

An article about the Enzyme-linked Immunosorbent Assay:
https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-elisa.html

Cheers
Henrik



Time Code:
0:00 What is an ELISA?
2:00 Sandwich ELISA example
Category
Biochemistry
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